4R4H
Crystal structure of non-neutralizing, A32-like antibody 2.2c in complex with HIV-1 Env gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2005-12-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.357, 69.429, 328.576 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.727 - 4.280 |
R-factor | 0.3097 |
Rwork | 0.309 |
R-free | 0.31990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.779 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.900 |
High resolution limit [Å] | 4.280 | 4.280 |
Rmerge | 0.361 | |
Number of reflections | 7941 | |
<I/σ(I)> | 2 | |
Completeness [%] | 76.8 | 65 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 10% PEG 5000 MME, 100 mM sodium acetate, and 100 mM Tris-HCl pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |