4R4H
Crystal structure of non-neutralizing, A32-like antibody 2.2c in complex with HIV-1 Env gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.357, 69.429, 328.576 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.727 - 4.280 |
| R-factor | 0.3097 |
| Rwork | 0.309 |
| R-free | 0.31990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.900 |
| High resolution limit [Å] | 4.280 | 4.280 |
| Rmerge | 0.361 | |
| Number of reflections | 7941 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 76.8 | 65 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 10% PEG 5000 MME, 100 mM sodium acetate, and 100 mM Tris-HCl pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
