4R4G
Crystal structure of a putative lipoprotein (ycdA) from Bacillus subtilis subsp. subtilis str. 168 at 2.62 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97971,0.91837,0.97926 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.653, 98.653, 195.018 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.327 - 2.620 |
R-factor | 0.2147 |
Rwork | 0.213 |
R-free | 0.23890 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XSCALE (January 10, 2014 BUILT=20140307) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.327 | 2.710 | |
High resolution limit [Å] | 2.620 | 5.610 | 2.620 |
Rmerge | 0.090 | 0.023 | 0.023 |
Rmeas | 0.031 | 2.396 | |
Number of reflections | 17632 | 1992 | 1638 |
<I/σ(I)> | 22.88 | 64.8 | 1.8 |
Completeness [%] | 99.8 | 99.3 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 50.0% polyethylene glycol 200, 0.1M TRIS pH 7.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |