4R4E
Structure of GlnR-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790, 0.97999, 0.9250 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 76.350, 103.300, 137.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.500 - 2.570 |
R-factor | 0.2543 |
Rwork | 0.254 |
R-free | 0.27460 |
Structure solution method | MAD |
Starting model (for MR) | Same crystal form but selenomet substituted. This starting model was used for final refinement with the 2.52 A native data |
RMSD bond length | 0.007 |
RMSD bond angle | 1.069 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 82.500 |
High resolution limit [Å] | 2.520 |
Number of reflections | 17350 |
<I/σ(I)> | 18.4 |
Completeness [%] | 98.0 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 40% PEG 400, 0.1 M CAPS, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |