4R48
Racemic crystal structure of a calcium-bound DNA four-way junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9998 |
Spacegroup name | C 1 2/c 1 |
Unit cell lengths | 65.950, 23.355, 72.907 |
Unit cell angles | 90.00, 115.47, 90.00 |
Refinement procedure
Resolution | 32.910 - 2.330 |
R-factor | 0.2887 |
Rwork | 0.285 |
R-free | 0.34090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dcw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.066 |
Data reduction software | XDS |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.910 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.044 | 0.293 |
Number of reflections | 4156 | |
<I/σ(I)> | 4.55 | 1.68 |
Completeness [%] | 95.2 | 94.34 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1 mM DNA, 75 mM sodium cacodylate, 30 mM calcium chloride, 1 mM spermine, 35% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |