4R3S
Crystal Structure of anti-MSP2 Fv fragment (mAb6D8)in complex with MSP2 11-23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-09 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.210, 64.340, 89.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.315 - 1.700 |
R-factor | 0.1543 |
Rwork | 0.152 |
R-free | 0.20040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyo |
RMSD bond length | 0.017 |
RMSD bond angle | 1.607 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.315 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.882 | 2.010 |
Number of reflections | 23184 | |
<I/σ(I)> | 5.15 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 33.8 | 32.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 30% PEG 8000, 0.2M NACL, 0.1M NAOAC, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |