4R3L

Crystal structure of Ard1 N-terminal acetyltransferase from Sulfolobus solfataricus bound to substrate peptide fragment and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A
Synchrotron site	NSRRC
Beamline	BL15A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-28
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	35.185, 58.004, 84.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.875 - 1.839
R-factor	0.1957
Rwork	0.190
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x7b
RMSD bond length	0.007
RMSD bond angle	1.090
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.839
Rmerge	0.044
Number of reflections	15532
Completeness [%]	99.8
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	283	12.5% PEG 4000, 0.1M sodium acetate trihydrate pH 4.2, 0.1M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 283K