4R3I
The crystal structure of an RNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-01-21 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.728, 39.748, 56.238 |
Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.2064 |
Rwork | 0.203 |
R-free | 0.26360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | a different crystal form of same protein |
RMSD bond length | 0.012 |
RMSD bond angle | 1.371 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.17) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.730 | 34.730 | 1.840 |
High resolution limit [Å] | 1.800 | 8.990 | 1.800 |
Rmerge | 0.112 | 0.041 | 0.587 |
Rpim | 0.027 | 0.369 | |
Total number of observations | 366 | 2551 | |
Number of reflections | 14029 | ||
<I/σ(I)> | 8.5 | 23.6 | 2.3 |
Completeness [%] | 97.1 | 92 | 90.7 |
Redundancy | 3.6 | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 25% PEG3350, 0.2 M amonium sulfate, 0.1 M bis-tris, pH 6.5, vapor diffusion, temperature 291K |