4R3F

Structure of the spliceosomal peptidyl-prolyl cis-trans isomerase Cwc27 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-10
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	58.613, 58.652, 59.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.979 - 1.300
R-factor	0.122
Rwork	0.121
R-free	0.14300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hq6
RMSD bond length	0.008
RMSD bond angle	1.246
Data reduction software	XDS
Data scaling software	SCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.980	1.330
High resolution limit [Å]	1.300	1.300
Number of reflections	49755
<I/σ(I)>	29.7	6.6
Completeness [%]	97.7	90.7
Redundancy	16.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291.15	0.1 M sodium acetate (pH 4.6) 8% PEG 4,000, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K