4R3E
Structure of the spliceosomal peptidyl-prolyl cis-trans isomerase Cwc27 from Homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-14 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 65 |
Unit cell lengths | 85.860, 85.860, 55.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.270 - 2.000 |
R-factor | 0.17398 |
Rwork | 0.172 |
R-free | 0.21716 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hq6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.084 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.270 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 30399 | |
<I/σ(I)> | 9.7 | 1.6 |
Completeness [%] | 99.3 | 93.5 |
Redundancy | 4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1 M magnesium formate 15% PEG 3,350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |