4R3E
Structure of the spliceosomal peptidyl-prolyl cis-trans isomerase Cwc27 from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 65 |
| Unit cell lengths | 85.860, 85.860, 55.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.270 - 2.000 |
| R-factor | 0.17398 |
| Rwork | 0.172 |
| R-free | 0.21716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hq6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.270 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30399 | |
| <I/σ(I)> | 9.7 | 1.6 |
| Completeness [%] | 99.3 | 93.5 |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1 M magnesium formate 15% PEG 3,350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
