4R38
LOV domain from Erythrobacter litoralis EL346 blue-light activated histidine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.081, 89.383, 122.404 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.117 - 1.600 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pr5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.289 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.057 | 0.042 | 0.624 |
| Number of reflections | 58404 | ||
| Completeness [%] | 95.7 | 98.4 | 64.4 |
| Redundancy | 5 | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 4000, 0.1 M HEPES, 0.1 M magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
