4R33

X-ray structure of the tryptophan lyase NosL with Tryptophan and S-adenosyl-L-homocysteine bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.8726
Spacegroup name	P 1 2 1
Unit cell lengths	94.720, 47.230, 114.360
Unit cell angles	90.00, 108.71, 90.00

Refinement procedure

Resolution	47.230 - 1.780
R-factor	0.1597
Rwork	0.159
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.230	1.890
High resolution limit [Å]	1.780	1.780
Number of reflections	179387
Completeness [%]	1.0	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	14-20% PEG 3350; 200 mM KBr; 1 mM Tryptophan; 15 mg/mL protein, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K