4R31

Crystal structure of a putative uridine phosphorylase from Actinobacillus succinogenes 130Z (Target NYSGRC-029667 )

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-03
Detector	RAYONIX MX225HE
Wavelength(s)	0.979310
Spacegroup name	P 21 21 21
Unit cell lengths	86.422, 120.595, 176.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.1713
Rwork	0.169
R-free	0.20810
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.344
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHELX (C)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.200
Number of reflections	126139
<I/σ(I)>	13.18	1.23
Completeness [%]	99.9	98.8
Redundancy	14.6	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Protein (20mM HEPES pH7.5, 150mM NaCl, 5% glycerol, and 5mM DTT); Reservoir (MCSG1 #81: 0.2 M sodium formate, 20% (w/v) PEG 3350); Cryoprotection (33% glycerol), Vapor Diffusion, Sitting Drop, temperature 298K