4R2Y
Crystal structure of APC11 RING domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 52.446, 39.876, 65.038 |
| Unit cell angles | 90.00, 108.49, 90.00 |
Refinement procedure
| Resolution | 61.681 - 1.755 |
| R-factor | 0.1954 |
| Rwork | 0.192 |
| R-free | 0.22910 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS-RAPD |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.681 | 1.850 |
| High resolution limit [Å] | 1.755 | 1.755 |
| Number of reflections | 24444 | |
| Completeness [%] | 94.6 | 80.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 16% PEG3350, 0.2 M NaNO3, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
