4R2K
Crystal structure of H119A mutant of YdaA (Universal Stress Protein E) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953725 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.820, 79.820, 262.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.460 - 1.970 |
| R-factor | 0.18969 |
| Rwork | 0.188 |
| R-free | 0.22857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 4R2J |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.857 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.910 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 36482 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil, Microbatch | 298 | 2M Ammonium sulfate, Under oil, Microbatch, temperature 298.0K |
