4R2J
Crystal structure of YdaA (Universal Stress Protein E) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.770, 85.770, 74.991 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.140 - 2.360 |
| R-factor | 0.21435 |
| Rwork | 0.212 |
| R-free | 0.26186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3OLQ |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.734 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.880 | 2.480 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 13536 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil, Microbatch | 5.8 | 298 | 0.2M Magnesium Nitrate hexahydrate, 20% PEG 3350, 10mM Adenosine 5'-(beta,gamma-imido)triphosphate, 0.2% n-dodecyl beta-D-maltoside, pH 5.8, Under oil, Microbatch, temperature 298.0K |
