4R2F
Crystal structure of sugar transporter ACHL_0255 from Arthrobacter chlorophenolicus A6, target EFI-510633, with bound laminaribiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-31 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 139.101, 139.101, 96.458 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.16636 |
Rwork | 0.165 |
R-free | 0.20695 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.364 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.115 | 0.970 |
Number of reflections | 36317 | |
<I/σ(I)> | 33.95 | 2.9 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 21.4 | 20.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 5% GLYCEROL, 5 MM DTT. RESERVOIR: 1.0 M AMMONIUM CITRATE TRIBASIC, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |