4R2E
Wilms Tumor Protein (WT1) zinc fingers in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.060, 78.301, 35.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.174 - 1.840 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.404 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.174 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.066 | 0.381 |
| Number of reflections | 16827 | |
| <I/σ(I)> | 23.1 | 3.5 |
| Completeness [%] | 99.3 | 94.2 |
| Redundancy | 6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 10% (w/v) PEG 6000, 5% MPD, 0.1 M Hepes-NaOH, pH 7.5 , VAPOR DIFFUSION, SITTING DROP, temperature 289K |
