4R2D

Egr1/Zif268 zinc fingers in complex with formylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	43.948, 55.912, 127.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.552 - 2.088
R-factor	0.1996
Rwork	0.198
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r2a
RMSD bond length	0.004
RMSD bond angle	0.635
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.557	2.160
High resolution limit [Å]	2.088	2.090
Rmerge	0.057	0.347
Number of reflections	9534
<I/σ(I)>	38.3	7.4
Completeness [%]	98.1	96.7
Redundancy	12.8	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	289	30% (v/v) PEG 400, 0.1 M CHES pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K