4R1U
Crystal structure of Medicago truncatula cinnamoyl-CoA reductase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792, 0.9184, 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.795, 96.821, 106.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.180 |
R-factor | 0.2022 |
Rwork | 0.198 |
R-free | 0.24240 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.180 |
Number of reflections | 38353 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 21% (w/v) polyethylene glycol 8000, 0.2 M potassium acetate, 100 mM HEPES, and 2 mM DTT, pH 7.5, vapor diffusion, hanging drop, temperature 277K |