4R1O
Crystal Structure of Thermophilic Geobacillus kaustophilus L-Arabinose isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00150 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.725, 140.746, 215.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.323 - 2.401 |
R-factor | 0.1669 |
Rwork | 0.164 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | GKAI with MN |
RMSD bond length | 0.007 |
RMSD bond angle | 1.091 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.115 | 0.458 |
Number of reflections | 133329 | |
<I/σ(I)> | 14.091 | 3.746 |
Completeness [%] | 96.2 | 94.1 |
Redundancy | 7.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 1.8M Na-K phosphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |