4R1J
Crystal structure of Arc1p-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2014-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97871 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.658, 49.961, 82.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.796 - 1.400 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.20130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fl0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.148 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.071 | 0.510 |
Number of reflections | 35696 | |
<I/σ(I)> | 13.3 | 3 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MES, pH 6.0, 10 % v/v glycerol, 30 % w/v PEG 600 and 5 % w/v PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |