4R1D
The crystal structure of Tle4-Tli4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.681, 135.179, 62.606 |
| Unit cell angles | 90.00, 113.49, 90.00 |
Refinement procedure
| Resolution | 20.003 - 1.749 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.21420 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.749 | 1.750 |
| Number of reflections | 86321 | |
| Completeness [%] | 94.4 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M BIS-TRIS pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
