4R12

Crystal structure of the gamma-secretase component Nicastrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0007
Spacegroup name	P 41 21 2
Unit cell lengths	65.584, 65.584, 344.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.076 - 1.950
R-factor	0.1807
Rwork	0.179
R-free	0.20550
Structure solution method	SAD
Starting model (for MR)	4UPC
RMSD bond length	0.021
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	56399
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1M Tris, 0.2M sodium chloride, 20%(w/v) PEG 3000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K