4R11
A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.057, 157.716, 84.824 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 44.686 - 2.789 |
R-factor | 0.2029 |
Rwork | 0.199 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.740 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.789 | 6.030 | 2.789 |
Rmerge | 0.073 | 0.032 | 0.655 |
Number of reflections | 54788 | ||
<I/σ(I)> | 9.3 | ||
Completeness [%] | 99.3 | 99.3 | 95.9 |
Redundancy | 3.6 | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 17% PEG 3350, 0.1M Bis-Tris-Propane, 0.2M sodium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |