4R0Z
A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-24 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.225, 38.972, 101.128 |
Unit cell angles | 90.00, 116.67, 90.00 |
Refinement procedure
Resolution | 33.674 - 2.005 |
R-factor | 0.1987 |
Rwork | 0.196 |
R-free | 0.23420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1I7W |
RMSD bond length | 0.003 |
RMSD bond angle | 0.747 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.038 | 0.025 | 0.222 |
Number of reflections | 36385 | ||
<I/σ(I)> | 17.1 | ||
Completeness [%] | 93.0 | 97.3 | 81.2 |
Redundancy | 4.5 | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.3M sodium formate, 50mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |