4R0W
Vvtgvta, an amyloid forming segment from alpha synuclein, residues 70-76
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.895432 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.367, 4.823, 15.692 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 23.970 - 1.500 |
| R-factor | 0.2467 |
| Rwork | 0.244 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal beta strand |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 2.560 | 1.500 |
| Rmerge | 0.193 | 0.164 | 0.177 |
| Number of reflections | 650 | ||
| <I/σ(I)> | 5 | ||
| Completeness [%] | 93.9 | 98.1 | 88.3 |
| Redundancy | 3.1 | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | isopropanol, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
