4R0U
Tgvtava, an amyloid forming segment from alpha synuclein, residues 72-78
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.895432 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 15.584, 4.742, 25.806 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 25.490 - 1.380 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal beta strand |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.510 |
| High resolution limit [Å] | 1.380 | 2.390 | 1.400 |
| Rmerge | 0.151 | 0.130 | 0.229 |
| Number of reflections | 798 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 83.8 | 95.1 | 61.2 |
| Redundancy | 3.4 | 4.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M magnesium formate, vapor diffusion, hanging drop, temperature 298K |
