4R0O
Crystal structure of PEGylated plastocyanin at 4.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 116.140, 116.140, 172.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.035 - 4.200 |
| R-factor | 0.2304 |
| Rwork | 0.229 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w88 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.470 | 4.310 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.350 | 0.011 |
| Number of reflections | 9064 | |
| <I/σ(I)> | 5.4 | 1.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293.15 | 48 % AMMONIUM SULPHATE, 30 mM POTASSIUM FERRICYANIDE, 100 mM SODIUM ACETATE, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
