4R0G

Crystal structure of Lpg0393 from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2013-04-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	79.636, 111.706, 167.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.039 - 2.700
R-factor	0.2239
Rwork	0.222
R-free	0.26890
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.400	2.700
Number of reflections	43800
<I/σ(I)>	24.5	2.7
Completeness [%]	99.7	99.7
Redundancy	6.1	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.15	8% PEG 8000, 0.1M HEPES, 8% ethylene glycol, 13% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K