4R0G
Crystal structure of Lpg0393 from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.636, 111.706, 167.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.039 - 2.700 |
R-factor | 0.2239 |
Rwork | 0.222 |
R-free | 0.26890 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.268 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.400 | 2.700 |
Number of reflections | 43800 | |
<I/σ(I)> | 24.5 | 2.7 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | 8% PEG 8000, 0.1M HEPES, 8% ethylene glycol, 13% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |