4R0C
Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-26 |
Detector | ACSD |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.145, 200.984, 101.493 |
Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
Resolution | 48.434 - 2.963 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.25020 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.351 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.090 |
High resolution limit [Å] | 2.960 | 5.920 | 2.960 |
Rmerge | 0.081 | 0.040 | 0.543 |
Number of reflections | 74781 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 96.7 | 97 | 92.1 |
Redundancy | 1.9 | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 30% PEG400, 0.05M Mg(Ac)2, 0.1M NaAc(5.0), and 3% glycerol, pH 7.5, vapor diffusion, temperature 298K |