4R0B
Structure of dimeric human glycodelin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 57.003, 57.003, 198.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.500 - 2.450 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.25660 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.175 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.550 |
High resolution limit [Å] | 2.450 | 10.000 | 2.450 |
Rmerge | 0.071 | 0.027 | 0.800 |
Number of reflections | 7678 | 146 | 835 |
<I/σ(I)> | 26.51 | 61.98 | 3.91 |
Completeness [%] | 99.9 | 95.4 | 100 |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25 %(w/v) PEG4000, 170 mM ammonium sulfate, 15 %(v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |