4R08
Crystal structure of human TLR8 in complex with ssRNA40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2012-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.320, 139.690, 167.570 |
Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
Resolution | 40.980 - 2.400 |
R-factor | 0.19322 |
Rwork | 0.190 |
R-free | 0.25758 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.482 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.220 |
High resolution limit [Å] | 2.400 |
Number of reflections | 143914 |
<I/σ(I)> | 8.8 |
Completeness [%] | 93.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 12-14% (w/v) PEG 3350, 0.2-0.3M potassium formate, 0.1M sodium citrate pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |