4QZG
Mouse Tdt, F401A mutant, in complex with a DSB substrate, C-C base pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.072270 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.992, 70.851, 114.893 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 42.480 - 2.750 |
| R-factor | 0.1887 |
| Rwork | 0.188 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i2a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.250 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.083 | 0.786 |
| Number of reflections | 11937 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
