4QZE
Mouse Tdt, F401A mutant, in complex with a DSB substrate, C-G base pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.872600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.033, 71.608, 113.948 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 40.840 - 2.250 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i2a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.045 | 0.640 |
| Number of reflections | 22175 | |
| <I/σ(I)> | 18.6 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
