4QYZ

Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2131
Spacegroup name	P 31 2 1
Unit cell lengths	223.869, 223.869, 290.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.473 - 3.030
R-factor	0.2257
Rwork	0.224
R-free	0.26720
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.524
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.473	3.050
High resolution limit [Å]	3.030	3.030
Number of reflections	163355
Completeness [%]	99.8	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	298	0.1 M sodium cacodylate, 0.1 M calcium acetate, 10% PEG 8,000, pH 5.0, VAPOR DIFFUSION, temperature 298K