4QYO
Crystal Structure of anti-MSP2 Fv fragment (mAb6D8)in complex with MSP2 14-22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.941, 60.712, 43.703 |
| Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
| Resolution | 41.864 - 1.208 |
| R-factor | 0.1521 |
| Rwork | 0.151 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3GM0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.864 | 1.251 |
| High resolution limit [Å] | 1.208 | 1.208 |
| Number of reflections | 62158 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 96.8 | 83.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 22% PEG 8000, 0.2M NaCl, 0.1M NaOAc, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
