4QXT
Crystal Structure of anti-MSP2 Fv fragment (mAb6D8)in complex with FC27-MSP2 14-30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.097, 60.213, 43.437 |
| Unit cell angles | 90.00, 107.12, 90.00 |
Refinement procedure
| Resolution | 34.388 - 1.580 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qyo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.180 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.157 | |
| Number of reflections | 28505 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 30% PEG 8000, 0.2M NaCl, 0.1M NaOAc, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
