4QXL
Crystal Structure of FLHE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00506 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.425, 36.755, 111.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.906 - 1.512 |
R-factor | 0.2012 |
Rwork | 0.200 |
R-free | 0.22550 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.540 |
High resolution limit [Å] | 1.510 | 4.100 | 1.510 |
Rmerge | 0.063 | 0.040 | 0.263 |
Number of reflections | 15949 | ||
<I/σ(I)> | 17.3 | ||
Completeness [%] | 92.3 | 97.9 | 80.2 |
Redundancy | 9.6 | 10 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.5 M sodium chloride, 10% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |