4QXL
Crystal Structure of FLHE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00506 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.425, 36.755, 111.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.906 - 1.512 |
| R-factor | 0.2012 |
| Rwork | 0.200 |
| R-free | 0.22550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.540 |
| High resolution limit [Å] | 1.510 | 4.100 | 1.510 |
| Rmerge | 0.063 | 0.040 | 0.263 |
| Number of reflections | 15949 | ||
| <I/σ(I)> | 17.3 | ||
| Completeness [%] | 92.3 | 97.9 | 80.2 |
| Redundancy | 9.6 | 10 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.5 M sodium chloride, 10% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
