4QXE

Crystal structure of LGR4 fused with hagfish VLR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2013-04-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 21 3
Unit cell lengths	173.759, 173.759, 173.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.21003
Rwork	0.209
R-free	0.23733
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v44
RMSD bond length	0.006
RMSD bond angle	1.123
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.290
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.116
Number of reflections	44070
<I/σ(I)>	4.5
Completeness [%]	100.0	10.29	69.61
Redundancy	28.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M HEPES pH 7.5, 2.0M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 289K