4QWQ
Crystal structure of the DNA-binding domain of the response regulator SaeR from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-04-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.974, 62.648, 116.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.324 - 2.501 |
R-factor | 0.2087 |
Rwork | 0.205 |
R-free | 0.28060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.106 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 8586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 285 | 15% PEG 6K, 0.1M Spermidine, 0.1M Bis-Tris PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 285K |