4QWQ

Crystal structure of the DNA-binding domain of the response regulator SaeR from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-04-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	31.974, 62.648, 116.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.324 - 2.501
R-factor	0.2087
Rwork	0.205
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.106
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	8586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	285	15% PEG 6K, 0.1M Spermidine, 0.1M Bis-Tris PH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 285K