4QVK

Apo-crystal structure of Podospora anserina methyltransferase PaMTH1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97626, 0.98401, 0.98004, 0.97989
Spacegroup name	P 21 21 21
Unit cell lengths	73.384, 79.719, 84.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.267 - 1.970
R-factor	0.1752
Rwork	0.174
R-free	0.20640
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.264
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	84.390
High resolution limit [Å]	1.970
Rmerge	0.067
Number of reflections	35566
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	20% PEG 3350, 0.1M Bis-Tris, 0.2M NaCl, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K