4QVD
E.coli Hfq in complex with RNA Ads
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97922 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.280, 68.270, 111.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.130 - 1.972 |
R-factor | 0.18699 |
Rwork | 0.185 |
R-free | 0.22923 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.256 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.190 | 2.080 |
High resolution limit [Å] | 1.972 | 1.972 |
Rmerge | 0.626 | |
Number of reflections | 29189 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 92.0 | 91.2 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 281 | 16% MPEG5000, 0.1M Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 281K |