4QVC
E.coli Hfq in complex with RNA Aus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.236, 67.989, 111.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.990 - 1.990 |
| R-factor | 0.2132 |
| Rwork | 0.211 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.070 |
| High resolution limit [Å] | 1.990 | 4.310 | 2.000 |
| Rmerge | 0.105 | 0.049 | 0.512 |
| Number of reflections | 29582 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 93.6 | 97.9 | 99.9 |
| Redundancy | 8.1 | 7.9 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 281 | 12% PEG4000, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
