4QV2
Unliganded crystal structure of Feline Norovirus P Domain co-crystallized with HBGA A-trisaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979701 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.480, 99.380, 59.890 |
Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
Resolution | 27.388 - 1.680 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.18310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.923 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.400 | 1.670 | |
High resolution limit [Å] | 1.630 | 7.270 | 1.630 |
Rmerge | 0.147 | 0.012 | 1.200 |
Rmeas | 0.037 | 1.404 | |
Number of reflections | 83120 | 943 | 5680 |
<I/σ(I)> | 9.45 | 34.63 | 1.28 |
Completeness [%] | 99.2 | 96.1 | 91.8 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, Glycerol, 2-propanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |