4QV2
Unliganded crystal structure of Feline Norovirus P Domain co-crystallized with HBGA A-trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979701 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.480, 99.380, 59.890 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 27.388 - 1.680 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.18310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.400 | 1.670 | |
| High resolution limit [Å] | 1.630 | 7.270 | 1.630 |
| Rmerge | 0.147 | 0.012 | 1.200 |
| Rmeas | 0.037 | 1.404 | |
| Number of reflections | 83120 | 943 | 5680 |
| <I/σ(I)> | 9.45 | 34.63 | 1.28 |
| Completeness [%] | 99.2 | 96.1 | 91.8 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, Glycerol, 2-propanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
