4QUZ
Crystal structure of Feline Norovirus P Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979738 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.740, 99.920, 59.610 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 49.634 - 2.350 |
| R-factor | 0.2169 |
| Rwork | 0.214 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.634 | 2.160 | |
| High resolution limit [Å] | 2.100 | 9.410 | 2.100 |
| Rmerge | 0.204 | 0.047 | 0.765 |
| Rmeas | 0.057 | 0.956 | |
| Number of reflections | 37583 | 409 | 2137 |
| <I/σ(I)> | 5.26 | 18.14 | 1.38 |
| Completeness [%] | 96.6 | 87.2 | 74.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, Glycerol, 2-propanol, Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
