4QUW
Crystal structure of the apo form of cyanobacterial aldehyde-deformylating oxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.682, 61.682, 110.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.150 - 2.260 |
| R-factor | 0.22857 |
| Rwork | 0.227 |
| R-free | 0.26576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oc5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 10534 | |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris hydrochloride pH 8.5, 30% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
