4QUF
crystal structure of chromodomain of Rhino with H3K9me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.317, 79.932, 81.181 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 37.216 - 2.501 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4quc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.131 | 0.818 |
| Number of reflections | 18675 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M ammonium nitrate and 20% PEG3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
