4QUC
Crystal structure of chromodomain of Rhino
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.029 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 36.480, 36.480, 76.115 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.589 - 1.502 |
| R-factor | 0.2131 |
| Rwork | 0.211 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kne |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.058 | 0.653 |
| Number of reflections | 9293 | |
| <I/σ(I)> | 61.9 | 3.1 |
| Completeness [%] | 94.0 | 85 |
| Redundancy | 10.3 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M Sodium nitrate and 20%(w/v) PEG3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
