4QU7

Crystal structure of a G-rich RNA sequence binding factor 1 (GRSF1) from Homo sapiens at 2.50 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	52.540, 72.320, 103.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.507 - 2.500
R-factor	0.1948
Rwork	0.192
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wez
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.507		2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.116	0.021	0.879
Rmeas		0.026	1.052
Number of reflections	14045	195	982
<I/σ(I)>	9.6	37.2	1.6
Completeness [%]	98.3	95.1	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20.0% polyethylene glycol 6000, 0.1M TRIS pH 8.5, 1mM AGGGAU, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K