4QU6

Crystal structure of a G-rich RNA sequence binding factor 1 (GRSF1) from Homo sapiens at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 42 21 2
Unit cell lengths	60.480, 60.480, 59.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.766 - 1.750
R-factor	0.1855
Rwork	0.183
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2db1
RMSD bond length	0.012
RMSD bond angle	1.548
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.766		1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.105	0.014	0.014
Rmeas		0.030	1.451
Number of reflections	11466	163	738
<I/σ(I)>	17.73	63.2	1.6
Completeness [%]	98.7	98.8	88.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M calcium chloride 20.0% polyethylene glycol 3350, 1 mM AGGGUG, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K